Journal: Eplasty
Article Title: Upregulation of Defensins in Burn Sheep Small Intestine
doi:
Figure Lengend Snippet: Longitudinal image of villi to demonstrate the localization of the antimicrobial SBD-3 (red) in the epithelial cells of the small intestine. Blue (DAPI) indicates cell nuclei and green (Phallicidin) shows F-actin. Note the green microvillous, brush border on the apical side of the cells (magnification ×200; deconvolution via 5 iterations). Panel A is magnification ×1200, Panel B is magnification ×500, and Panel C is magnification ×500. White arrows indicate SBD-3 in the epithelium and subepithelial capillaries in the panel A and are associated with the lacteal and surrounding capillaries in panel C. Scale bar in panel B = 90 microns.
Article Snippet: Deconvolution and image analysis were performed by transferring the data sets to a Linux/RedHat workstation employing SoftWoRx software (Applied Precision), which uses an algorithm experimentally produced on the system from the convolution of a point spread function (PSF) to differentiate and reduce extraneous light or scattered light captured by the camera.
Techniques: